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Further investigation revealed that the use of fatty di-acids longer than 14 carbons attached via Glu resulted in loss of activity, while the use of mono-acids of up to 16 carbons (palmitate) retained activity, as shown in Table 1

RNA-seq analysis was performed using three biological replicates per condition ( b, c )

To identify the treatment cohort, the KRD was searched for all patients with 1 claim for semaglutide 2.4 mg between June 15, 2021 and July 31, 2022