After washing and resuspension in HBSS containing 0.1% BSA and metabolic inhibitors (20 mmol/L 2-deoxygucose and 10 mmol/L NaN 3 ) to prevent GLP-1R internalisation (Widmann et al., 1995), binding experiments were performed using FITC-conjugated ligands as described below, with TR-FRET measured in a Flexstation 3 plate reader (Molecular Devices) using the following settings: ex = 335 nm, em = 520 and 620 nm, delay 50 s, integration time 400 s
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It may be recorded as an associated identifier, but it should not replace the exact catalogue name
Wegovy tablets offer a needle-free approach to weight loss, expanding the number of people who can access life-changing treatment
You should finish each set feeling like you could have done one or two more, but not five more