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As previously described (Strbe et al., 2021), intestinal tissue damage was analyzed using a histologic scoring scale adapted from Chui and coworkers (Chui et al., 1970) on a scale of 05 (normal to severe) in three categories (mucosal injury, inflammation, and hyperemia/hemorrhage) for a total score of 015, as described by Lane and coworkers (Lane et al., 1997)

For keeping up the treatment, injections are often given monthly

Nat Rev Cancer (2002) 2(6):44254

The concentration of Mouse SCF in the samples is then determined by comparing the OD of the samples to the standard curve

Sperm DNA methylation is predominantly stable in mice offspring born after transplantation of long-term cultured spermatogonial stem cells