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Deeply elucidating molecular anti-aging mechanisms, investigating interactions with other aging-related signaling pathways (such as mTOR, AMPK, etc.), improving the functional network, and providing theoretical support for more efficient anti-aging interventions

After a wash in TBS for 10 min, slides were incubated for 45 min in Flex+Linker mouse (Dako) or Post Primary (Leica, Zrich, Switzerland), then washed again in TBS, and incubated for 45 min in Flex+HRP (Dako) or Polymer (Leica)

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