Furthermore, the 5xFAD mouse, which overexpresses human APP with three mutations along with human presenilin 1 (PS1) harboring two mutations, begins to show significant apathy-like behaviors from six months of age, as measured by nest-building, burrowing, and marble burying (Keszycki et al., 2021
The monoiodoacetate model of osteoarthritis pain in the mouse
This reaction breaks the disulfide bonds, converting them into two thiol groups, and facilitates targeted drug release where it is needed most
Yang and Stockwell, 2008)
Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)