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Subsequently, the samples were incubated with 3% H 2 O 2 to remove endogenous peroxidase activity and blocked with BDT (TBS containing 3% BSA and 10% goat serum) for 30 min, followed by overnight incubation with primary antibodies at 4 C
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![Mechanism of action of GLP-1 [25]](https://www.researchgate.net/publication/256289222/figure/fig1/AS:614177232142337@1523442671231/Mechanism-of-action-of-GLP-1-25.png)
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