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The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates
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In PD neurons, abnormal -synuclein deposition is accompanied by compensatory upregulation of the mitochondrial protease LONP1, suggesting that activation of the mitochondrial quality control system may serve as a critical defense mechanism against proteotoxic stress [97]
PMC 24216
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