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This conjugate is metabolized by -glutamyltranspeptidase into sulforaphane-cysteine-glycine, then by cysteinyl-glycinase to form sulforaphane-cysteine, and finally by N-acetyltransferase to form sulforaphane N-acetylcysteine

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WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA

10.1007/s10541-005-0132-1 48 ShilovskyG