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A Certificate of Analysis confirming purity and molecular identity is available per lot

Melanotan I Class: Peptide analogue (research use) Molecular formula (free base): C78H111N21O19 Molecular weight (free base): ~1646.9 g/mol CAS: 75921-69-6 Form: Lyophilised powder Appearance: White to off-white solid Handling & Storage Storage (dry): Keep vials protected from light and moisture

Cells were incubated overnight in INS-1 832/3 media at 3 mM glucose 24 hours after cDNA and 48 hours after siRNA transfections and then pre-incubated for 30 minutes in KRBH buffer supplemented with 3 mM glucose before stimulation in KRBH buffer supplemented with 11 mM glucose 100 nM exendin-4 for 60 minutes