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The complex was solubilized from membrane using 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG) and 0.03% (w/v) cholesteryl hemisuccinate tris salt (CHS) (Anatrace) for 1h at 4 C
In the mid 1980s, John Eng was studying bioactive molecules in the venom of the Gila monster (Heloderma suspectum), a lizard native to the southwestern United States and Mexico
However, its best used with medical oversight
Eur J Clin Microbiol Infect Dis
PVDF membranes were cut to size and blocked in 5% (w/v) non-fat milk powder dissolved in TBS-T (TBS-Tween 20 (0.01% v/v)) for at least 1 h at room temperature (RT)
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