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Nothing in the table is a recommendation about how much material to use in an experiment it is the arithmetic that tells you what concentration you are holding once you have added a known volume, so that the number you write in the notebook matches what is in the container

Examples may include the luteinizing hormone (LH), which is considered a crucial hormone in the regulation of reproductive hormones such as testosterone

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Then, the cells were trypsinised, fixed with 4% PFA, permeabilised and labelled with Alexa Fluor 647 picolyl azide