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Thermal shift assay (TSA) Samples were prepared by using a final concentration of 0.25 mg/ml TGR diluted in TGR reaction buffer to the desired volume

Through oligomerization, ninjurin-1 causes the plasma membrane to rupture completely, resulting in the efflux of large damage-associated molecular patterns (161, 162)

Blood and skeletal muscle (mainly composed of gastrocnemius muscle) samples were collected from all animals

Individual results vary depending on skin type and routine consistency

illustrated how the selective permeability of the blood-brain-barrier (BBB) and bloodCSF barrier, combined with the spatial distribution of glutathione-metabolizing enzymes, underlies region-specific vulnerability to oxidative injury in the CNS (9)