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Hyperglycemia-induced activation of the hexosamine pathway further dysregulates protein glycosylation and endothelial homeostasis (50, 51), while PKC, particularly the -isoform, promotes vascular leakage, basement membrane thickening, and VEGF-driven neovascularization through NF-B and VEGF upregulation (5254)

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The pAd shRNA plasmids were then transfected into HEK 293A cells (Invitrogen, Carlsbad, CA, United States) to generate adenovirus, after P ac I digestion