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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate

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PZA and INH treatment to in vitro granulomas generated from T2DM subjects at 3-months post- L-GSH supplementation also resulted in a significant decrease in the production of IL-6 when compared to before L-GSH supplementation in the untreated (control) category ( Figure 7A )