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Each puncture introduces a contamination event, and benzyl alcohol inhibits growth of introduced organisms but cannot sterilize the solution if contamination has already occurred

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Following PBST washes, biotinylated secondary antibodies were applied, followed by ABC reagent (Vector Laboratories, Inc., Newark, CA, USA, #PK-400) and DAB substrate (Vector Laboratories, Inc., #SK-4105)

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The 'take it whenever' advice that works for metformin fails here