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Acceptor photobleaching FRET To determine the FRET Efficiency of the Ent1/2p-mNeonGreen/Chc-mScarlet pair, yeast cells previously described were grown overnight at 30 C with shaking in a 24-well plate using LD(=low-fluorescence SD)-Trp - , Leu - medium (Yeast Nitrogen base without aminoacids supplemented with the corresponding DropOut media, Formedium, CYN402)

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