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Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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Some of the most common are: Anemia, when the body does not have enough red blood cells Thyroid issues Depression Any chronic condition or injury that causes persistent pain Sleep disorders Addisons disease, when the adrenal glands dont produce enough hormones Eating disorders Viral illnesses, like the flu or COVID-19 Diabetes Kidney disease Liver disease A healthcare provider can diagnose the underlying cause of fatigue, whether its semaglutide or another underlying condition

Davies MJ, Bajaj HS, Broholm C, et al