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And, you know, interesting other pathways these days, what I really recommend if you're going to do NAD is also really thinking about the two salvage pathways that NAD can break down into

Media (ADF plus 20% FCS) was changed every 2 days, and cells were split twice a week

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It also explains why B12 deficiency symptoms (like fatigue, brain fog, numbness, or mood changes) can take time to return once levels are restored

Watch out for irritation, dryness, redness, or sensitivity