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Immunoblotting was performed with 10 g of cytoplasmic and mitochondrial-enriched lysate as per the immunoblotting methods described below relative to whole-cell lysates
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Additionally, the serine protease dipeptidyl peptidase 4 (DPP4) limits the bioavailability of GIP, GLP-1, and GLP-2 by cleaving the first two amino acids, rendering them inactive (Knudsen and Pridal, 1996
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