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The mutations in certain genes such as PTEN , fragile X mental retardation 1 ( FMR1 ), chromodomain helicase DNA binding protein 7 ( CHD7 ), and tuberous sclerosis 1 and 2 ( TSC1 and TSC2 ) are associated with the ASD [208,209,210,211,212]

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To address this, we generated double deletion strains lacking both gshA and either the gene coding for the periplasmic binding protein GsiB, or one of the two permease domains coding genes gsiC or gsiD