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Therefore, the primary aim of this study was to assess the timeline over which changes in both subjective feelings of appetite and appetite-related hormones are activated during WL with a ketogenic VLED

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coli, the codon-optimized gene sequence was cloned between the Nde I and Apa I restriction sites of the pET30a+ vector using the Snapgene tool ( (accessed on 16 August 2025)

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