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This is likely due to protein misfolding caused by the initial carbonylation of the surface residues and subsequent exposure to ROS of the previously hidden parts of the polypeptide

The fractional glucagon release in isolated islets was 0.390.06 %/h (mean value SEM of 15 preparations using 150 mice)

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Slices were then incubated for 10 min at 34 C (monitoring of bubbling, keeping slices in motion using a pipette) in wells containing 600 L of aCSF at 34 C (including 1 mM or 5 mM glucose) under fine bubble carbogen perfusion (1 Hz)