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NAD+ (nicotinamide adenine dinucleotide) is a coenzyme important for cellular energy production, DNA repair, and sirtuin activation

Existing studies are generally small in sample size and limited in follow-up duration

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Multiple tumour areas in the large intestine were quantified and demonstrate a similar increase in mitochondrial content when treated with 5FU (Extended Data Fig

For the cell experiments, all cell samples were randomly coded by an independent third party, and experimenters conducted treatments and measurements without knowledge of the specific group assignments