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The improved stability of the arginate form has profound implications for study design and potential outcomes

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[73] As noted above, the completely synthetic laboratory synthesis of B 12 was achieved by Robert Burns Woodward and Albert Eschenmoser in 1972, though this process has no commercial potential, requiring more than 70 steps and having a yield well below 0.01%

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The unamidated free-carboxylic-acid form binds with substantially reduced affinity and produces a different downstream signalling profile in cell-based assays