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The gradual dose escalation protocol specifically aims to minimize these effects while building therapeutic benefit
In contrast, DIO mice in both groups switched from WD to LD each had significantly increased Proteobacteria relative abundance (represented solely by the genus Parasutterella ) compared to both the WD and the LD controls, suggesting that Proteobacteria relative abundance may be largely influenced by stress or the differences in net energy expenditure (or associated body weight changes) caused by the diet switch
Preclinical studies using T4 (including the RGN-259 ophthalmic clinical trials) have not identified significant adverse events at therapeutic doses
Based on this, anecdotal sources report a dose of 10-20mcg/kg, which would be equal to 1-2mg for a 200lb test subject
References Adil M, Visnagri A, Kumar VS, Ghosh P (2014) Protective effect of naringin on sodium arsenite induced testicular toxicity via modulation of biochemical perturbations in experimental rats
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