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Briefly, three different solutions were prepared as follows: A A solution comprising 2% sodium carbonate (Na 2 CO 3 ) dissolved in 0.1 normal (N) sodium hydroxide (NaOH)

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Although these caspases cannot directly cleave the precursors of IL-1/IL-18, they can still trigger pyroptosis by cleaving GSDMD-NT, a process specifically activated by bacterial lipopolysaccharides [67, 68]