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Circulating Homocysteine and Folate Concentrations and Risk of Type 2 Diabetes: A Retrospective Observational Study in Chinese Adults and a Mendelian Randomization Analysis. Frontiers in Cardiovascular Medicine, vol
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Each sample was then split into two fraction (100 L) and treated as follows: For H 2 S measurement, 100 L of each sample was precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 L of 50 mM HEPES buffer (pH 7.4)
All quantified proteins with non-missing values were included in the ranking
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