1 Introduction DOR is characterized by a reduction in the number and/or quality of oocytes, leading to insufficient ovarian function, sex hormone deficiency, and decreased fertility
Proper preparation and storage help minimize unwanted variations in response
All experiments were conducted in a buffer solution containing 20 mM HEPES (pH 7.0), 150 mM NaCl, 1 mM dithiothreitol (DTT), and 5% dimethyl sulfoxide (DMSO)
If periods are erratic, or sperm count is low, pregnancy may start to feel like a challenging goal
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