sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
Lipotropic (MIC)+B12 injections are typically administered intramuscularly (IM) or subcutaneously (SQ), depending on your clinicians instructions and the specific formulation you receive
doi: 10.1002/prot.22711 169 MartinekTDubou-DijonETimrMasonPEBaxovKFischerHEet al
You may feel a small pinch at the injection site
No they're complementary rather than substitutable