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were produced using 5ug/mL of (13)--D-glucan from heat killed Candida albicans for 24 hours, as previously described ( in vitro experiments were stimulated with 5g/mL of (13)-B-D-glucan from Alcaligenes faecalis (Sigma-Aldrich), 100ng/mL of lipopolysaccharide (LPS) from Escherichia coli O127:B8 (Sigma-Aldrich), 120M L-buthionine sulfoximine (Sigma-Aldrich), and/or 1mg/mL of reduced glutathione (Sigma-Aldrich) for 24 hours unless otherwise stated in figure legends

doi:10.1016/j.nmni.2021.100897 Kobayashi, Akie et al

Some retention is expected, but dramatic puffiness or rapid weight gain from fluid warrants dose reduction

Front Immunol (2018) 9:2251

A vial left in the freezer and only removed once for use will retain full potency