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For Th17 assay, cells were stimulated with Cell Stimulation Cocktail (eBioscience, USA) for 6 h at 37 C and 5% CO 2 and then stained with anti-mouse CD4-phycoerythrin (PE) (#12-0041-82, eBioscience, USA)
These criteria included moderate to severe obesity (BMI between 30 and 39.99), a waist circumference greater than 94 cm, total testosterone (TT) levels below 12 nmol/L, presence of sexual symptoms, normal-to-low gonadotropin levels (within the lowest quartiles of the reference range), SHBG levels within the lower quartile of the reference range, and a Homeostasis Model Assessment Index (HOMA) greater than 2.5
Zhou X et al
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