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2 Takeda Development Center Americas, Inc., Lexington, MA, USA at the time of the study
Lentivirus Production and Transduction TSA201 cells were transfected with the Grx1-roGFP2 encoding expression plasmid pGIPZ-CMV-Grx1-roGFP2, packaging plasmid psPAX2 (Addgene plasmid #12260) and envelope plasmid pMD2.G (Addgene plasmid #12259
The peptide is not damaged by extra water, just more dilute
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Highly purified water (typically water for injection, which has undergone multiple distillation or reverse osmosis) is mixed with a precisely measured amount of benzyl alcohol so that the resulting concentration is exactly 0.9% (weight/volume)
