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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h
Our model simulations are reflective of the hepatic GSH environment, agreeing with the supra-pharmacological concentrations of doxorubicin required to illicit comparable GSH depletion in the liver 39
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Thus, co-administration with CF correctors or potentiators would be necessary
Nature 434 , 113118 (2005)
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