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The tissues were analyzed on the Ventana Discovery Ultra automation system (Ventana Medical Systems) for GLP-1 receptor expression using rabbit-anti-mGLP1R (Abcam, ab218532, lot: GR3231665-2) at a concentration of 2.7 g ml 1
It peaks in the morning and declines throughout the day
Then we evaluated the dendriplex PURE G4 -LA 12 -anti- GLS1 -siRNA effect directly on GBM cells and after crossing two in vitro models of the BBB
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