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If your research hypothesis involves dose-response relationship testing, include at least three dose groups (low, medium, high) with vehicle control rather than escalating within a single group

Also, autaptic hippocampal cultures produced from these transgenic mice showed a decrease in both amplitude and frequency of EPSCs, whilst neurons of Mecp2 Tg1 mice, with an upregulated Mecp2 gene, had an opposite effect [377]

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[PubMed]^6 U.S

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