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After incubation, supernatants were taken, remaining cellular debris removed by centrifugation (200 g , 5 min, 4 C), and the amount of ATP was measured using the CellTiter-Glo 2.0 (Promega) ATP kit and a Tecan Spark plate reader

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for example, Light-medium brown skin: 1-3 months, Dark brown skin: 3-6 months, very dark skin: 6-12 months & Black skin: 18 months minimum

Side effects: Lower doses do not eliminate the possibility of nausea, fatigue, dehydration, or blood sugar changes

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