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Heat-induced epitope retrieval (HIER) was performed in a pressure cooker for 20 min using either citrate-based buffer at pH 6.0 (VectorLab, H-3300-250) or tris-based buffer at pH 9.0 (VectorLab, H-3301-250), according to the antibody manufacturers instructions

Garden of Life mykind Organic Whole Food B-12 Spray: Each 1 mL spray contains 20,833% of the DV for vitamin B12 along with a certified organic blend of fruits, vegetables, and herbs

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Here the Intrinsic Factor binds to the B12 and the "B12/IF Complex" enters the cells on the wall of the Ileum after binding to receptors on the surface of the Ileal cells, allowing it to enter the blood stream