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Always follow your laboratory's protocol or the manufacturer's documentation when determining reconstitution volumes
Its sequence is Gly-Glu-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV)
The chosen volume is a research-handling decision, not a fixed rule so verify it with the peptide calculator
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Comparison with structures of the AMY 1 R bound to non-lipidated peptides (CagriBB and rAmy) revealed that the lipid conjugation does not affect the conformation of the peptide, nor the receptor when Cagri is stably engaged
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