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Real-time quantitative PCR (RT-qPCR) was performed in triplicate on a CFX96 system (Bio-Rad, Hercules, CA, USA) using SYBR Green Mix (Toyobo, Osaka, Japan)

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Treatment of mouse heart homogenates generated under native conditions with Na 2 S 2 also led to the formation of PrSS and PrS(S) n S detected by the same procedures (Supplementary Fig

It has been previously reported that other lipases are activated or stabilized in the presence of bile salts, for example bile salt dependent lipase 62,63

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