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For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)
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Nle-AngIV also increased spine numbers to 32 spines/50m, but to a lesser extent than Dihexa
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Energy and metabolism improvements happen because glutathione protects your mitochondria - cellular powerhouses
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