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thaliana is not haploinsufficient for telomerase and plants with only 50% of the telomerase activity of wild type do not undergo telomere shortening ( Telomere shortening can occur from loss of chromosome end protection, triggering exonucleolytic degradation (Song et al., 2008
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Western blot and RT-qPCR As described previously [18], total protein extracted from lung tissues and HUVECs were electrophoresed on a 8 or 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel, electroblotted to a polyvinylidene difluoride membrane (Millipore, Billerica, MA), incubated with primary antibodies against DPP-4 (Abcam), GLP-1 (Abcam), VE-cadherin (Abcam), Fibronectin (Abcam), -SMA (Abcam), ZO-1 (ProteinTech Group, Inc., Chicago, IL, USA), vimentin, Smad2/3, p-Smad2/3, extracellular regulated protein kinases1/2 (ERK1/2), p-ERK1/2 (Cell Signaling Technology, MA, USA), or -actin (ProteinTech Group)
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