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It is extracted from Chinotto a fruit-bearing tree native to Southern China (hence its name) which was imported in Italy most probably around the VI and VII century A

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

DOAC kidney clearance varies (dabigatran 80%, edoxaban 50%, rivaroxaban 35%, apixaban 27%)