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For the IF-staining of the c-fos marker, brain sections (5 m) were incubated overnight at 4C in primary antibody (1:1,000, ab208942, abcam, UK) before being incubated with fluorophore-conjugated goat anti-mouse secondary antibody (1:1,000, ab150113, abcam, UK) for 30 min, then the CY3-Tyramide (1:500, G1223, Servicebio, China) was used for tyramide signal amplification

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