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Shaking the vial vigorously can cause mechanical stress that breaks the peptide bonds, a process known as shearing

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Vitamin B12 (also called cobalamin) promotes healthy nervous system function and can be added to your diet through shellfish, meat, poultry, eggs, milk, dairy, and fortified products like cereals, soy, and nutritional yeast

The specific concentration, exposure time, and experimental protocols are determined by the researcher based on the study design, existing literature, and institutional guidelines