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Briefly, cryopreserved terminally differentiated HepaRG cells (BioPredic International, Saint Grgoire, France) were cultured per manufacturer guidelines

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Statistical analysis Using data generated from a pilot study and G*Power 3.1 software (Dsseldorf, Germany), it was determined that a sample size of 15 would provide 80% power to detect an increase in CNS GSH concentrations between pre- and post-administration values, with an accepted alpha value of 0.2