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Estimation of the global prevalence of dementia in 2019 and forecasted prevalence in 2050: an analysis for the global burden of disease study 2019

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For the extraction of mRNA, tissue samples were homogenized in TRI Reagent (Molecular Research Center) using a TissueLyser II system (Qiagen) and then chloroform extracted, precipitated using isopropanol, washed with 75% ethanol, and reconstituted with DEPC-treated water