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Tissue culture media supernatants from the final 24 hours of culture were collected and assayed by Amplex Red Glucose/Glucose Oxidase Assay Kit (Life Technologies, Gaithersburg, MD, USA), Lactate Assay kit (Sigma-Aldrich) and Rat Ultrasensitive Insulin ELISA (Mercodia, Uppsala, Sweden) to measure total glucose, lactate and insulin respectively

In the entomopathogenic nematode Steinernema kraussei , GPC plays an important role in stress resistance by activating the expression of the daf-16 gene within the insulin/IGF-1 signalling (IIS) pathway [48]

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Aquaculture 249 , 439447 (2005)

While the Act does not target RUO products by name, its effect is clear