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Dilute solutions (2.5 mg/mL) need larger volumes, making precise measurements easier but requiring more storage space

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Very common (more than 1 in 10): mild pain, redness, or swelling at injection site usually fades in a day

After transfection of the sgRNA libraries into CAG-C9-3 cells, we obtained two stable cell populations carrying constitutively expressed Cas9 and sgRNAs (Trim-library-1 and Trim-library-2)