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Next, the time course of rebound in GSH levels after the CS exposure was examined
This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane
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showed "GSH was highly tolerated and its administration was not associated with any signs of adverse effects." [2] Blood chemistry markers, including liver and kidney function tests, remain unaffected even with extended use
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