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Because so many pathways are implicated at once, most research protocols isolate a small panel of markers, such as collagen I/III ratios or a single antioxidant enzyme, rather than tracking every reported change simultaneously

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Several amino acids had their concentrations increased following MTX treatment: glycine, alanine, phenylalanine, threonine, histidine, valine, arginine, leucine, isoleucine, tyrosine, and proline

doi:10.1515/CCLM.2007.362